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Addgene

p3E-MCS
(Plasmid #75174)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75174 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDONRP4P1R
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2646
  • Total vector size (bp) 2709
  • Vector type
    Three prime entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Multiple Cloning Site
  • Insert Size (bp)
    63
  • Promoter n/a

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The multiple cloning site insert was purchased from GeneArt™ Genestring™ Fragment (Invitrogen/ ThermoFisher Scientific)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3E-MCS was a gift from Nicholas Cole (Addgene plasmid # 75174 ; http://n2t.net/addgene:75174 ; RRID:Addgene_75174)
  • For your References section:

    A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease. Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ. Zebrafish. 2016 Sep 15. 10.1089/zeb.2016.1321 PubMed 27631880