pAcBac1-BacMam-4x-PyOtR
(Plasmid #217364)

• Purpose: Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid
• Depositing Lab: Abhishek Chatterjee
• Publication: Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202316428. doi: 10.1002/anie.202316428.

Backbone

• Vector backbone: pAcBac1
• Backbone manufacturer: derived from pFastBac-Dual from Invitrogen.
• Backbone size w/o insert (bp): 8024
• Total vector size (bp): 9411
• Vector type: Mammalian Expression, Insect Expression ; baculoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Gentamicin, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 4 copies of pyrrolysyl-tRNA mutant PyOtR
• Alt name: 4xU6-PytOR
• Alt name: 4xPytOR
• Species: Methanosarcina mazei
• Insert Size (bp): 1387
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AvrII (not destroyed)
• 3′ cloning site: AvrII (destroyed during cloning)
• 5′ sequencing primer: CCAATAGGCCGAAATCGGCAAAATCC
• 3′ sequencing primer: GCAGCTTATAATGGTTACAAATAAAGCAATAGC

Resource Information

• Supplemental Documents:
  • pacbac1-bacmam-4x-pyotr.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry