pOET5 rota-VLP
(Plasmid #218147)

• Purpose: Forms rotavirus-like particles in insect cells by expression of VP6 (GenBank ID: ACL93331.1).
• Depositing Lab: Minna Hankaniemi
• Publication: Plasmid-based production of noro, rota, and enterovirus-like particles in insect cells (unpublished)

Backbone

• Vector backbone: pOET5.1
• Backbone manufacturer: Oxford Expression Technologies
• Backbone size w/o insert (bp): 4581
• Total vector size (bp): 5775
• Modifications to backbone: P10 promoter replaced with CMV enhancer and promoter
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rotavirus VP6
• Alt name: Rotavirus capsid protein VP6
• Alt name: VP6
• Species: H. sapiens (human); Human rotavirus
• Insert Size (bp): 1194
• GenBank ID: ACL93331.1
• Promoter: Polyhedrin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CTGTTACGAAAACAGTAAAATACTT
• 3′ sequencing primer: TAAATCAACAACGCACAGAATCTAG

Resource Information

• Supplemental Documents:
  • pOET5-wt-VP6_ordered_010422.gbk
• A portion of this plasmid was derived from a plasmid made by: Gene synthetically produced and cloned by GenScript.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry