Pooled library screening with multiplexed Cpf1 library.
Liu J, Srinivasan S, Li CY, Ho IL, Rose J, Shaheen M, Wang G, Yao W, Deem A, Bristow C, Hart T, Draetta G
Nat Commun. 2019 Jul 17;10(1):3144. doi: 10.1038/s41467-019-10963-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127749 | LentiUniversal-Puro | Lentiviral plasmid for expressing any U6 driven transcripts (sgRNAs, crRNAs, shRNAs and etc...) |
| 127750 | LentiAsCpf1-3xMYC-blast | Lentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signal |
| 130630 | Mini-human AsCpf1-based human genome-wide knockout library | Expresses crRNAs for genome-wide knockout pooled library screening |