Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
A toolbox to engineer the highly productive cyanobacterium Synechococcus sp. PCC 11901.
Victoria AJ, Selao TT, Moreno-Cabezuelo JA, Mills LA, Gale GAR, Lea-Smith DJ, McCormick AJ
Plant Physiol. 2024 May 7:kiae261. doi: 10.1093/plphys/kiae261. PubMed Article

Plasmids from Article

ID Plasmid Purpose
203934pC0.420Up Flank. Neutral site. Up flanking sequence of type II site-specific deoxyribonuclease AquI (FEK30_10065) in Synechococcus sp. PCC 11901.
203935pC0.419Down Flank. Neutral site. Down flanking sequence of type II site-specific deoxyribonuclease AquI (FEK30_10065) in Synechococcus sp. PCC 11901.
203936pC0.385Up Flank. Neutral site. Up flanking sequence of ω3 acyl-lipid desaturase (desB, FEK30_04840) in Synechococcus sp. PCC 11901.
203937pC0.378Down Flank. Neutral site. Down flanking sequence of ω3 acyl-lipid desaturase (desB, FEK30_04840) in Synechococcus sp. PCC 11901.
203938pC0.386Up Flank. Neutral site. Up flanking sequence of glycogen synthase A1 (glgA1, FEK30_14880) in Synechococcus sp. PCC 11901.
203939pC0.387Down Flank. Neutral site. Down flanking sequence of glycogen synthase A1 (glgA1, FEK30_14880) in Synechococcus sp. PCC 11901.
203940pC0.388Up Flank. Neutral site. Up flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901.
203941pC0.389Down Flank. Neutral site. Down flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901.
203942pC0.437Up Flank. Neutral site. Up flanking sequence of intergenic region (NS1) between FEK30_11550 and FEK30_11555 in Synechococcus sp. PCC 11901.
203943pC0.438Down Flank. Neutral site. Up flanking sequence of intergenic region (NS1) between FEK30_11550 and FEK30_11555 in Synechococcus sp. PCC 11901.
203944pC0.425Up Flank. Up flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.
203945pC0.426Down Flank. Down flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.
203946pC0.422Level 0 part. Rhamnose-inducible rhaS_PrhaBAD promoter.
203947pC0.423Level 0 part. 2,4-diacetylphloroglucinol (DAPG)-inducible phlF_PphlF promoter.
203948pC0.391Level 0 part. Cas12a coding sequence from Francisella novicida in CDS1 position
203949pC0.393Level 0 part. Gentamicin resistance cassette in CDS2 position
203950pCA0.421Level 0 acceptor vector for assembling gRNA arrays for Cas12a in SP position
203951pCA1.339Spectinomycin-based RSF1010 acceptor Level 1
203952pCAT.482Spectinomycin-based RSF1010 acceptor Level T
203953pCA1.327Level 1 Position 1 pUC19 acceptor vector
203954pC0.394Level 0 part. theophylline responsive riboswitch E* promoter.
203955pC1.509Level 1 self-replicating vector containing a DAPG inducible FnCas12a
205441pC1.530Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.

Antibodies from Article