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Addgene

Human Genome-wide CRISPRi-v2 Libraries
(Pooled Libraries #83969, #1000000090)

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  • Purpose

    Version 2 - Human genome-scale CRISPRi library for inhibition of gene transcription. Library available as top 5 sgRNAs/gene.

  • Vector Backbone

    pCRISPRia-v2 - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 83969
  • One tube, contains single half-library, top 5 sgRNAs/gene†
1 $ 640 Add to Cart
Pooled Library 1000000090
  • Two tubes, each with a half-library in backbone pCRISPRia-v2†
    • #83969 (top 5 sgRNAs/gene)
    • #83970, an additional half-library at 5 sgRNAs/gene
 1
This material is available to academics and nonprofits only.
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with dCas9-KRAB (Addgene #46911) or otherwise used with cell lines already expressing inhibitory dCas9.

Library Details

  • Species
    Human
  • Genes Targeted
    18,905
  • gRNAs

    • #83969: 104,535
    • #1000000090: 209,070
  • Controls

    • #83969: 1,895
    • #1000000090: 3,790
  • Lentiviral Generation
    3rd

Library Shipping

These libraries are delivered as suspended DNA in microcentrifuge tubes on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.

Pooled Library #83969 will be delivered as a single tube containing a pooled half-library with 5 gRNAs per gene.

Discontinued: Pooled Library #1000000090 will be delivered as two tubes, each containing a pooled half-library with 5 gRNAs per gene (for a total of 10 unique gRNAs per gene).

  • Volume
    20 µL
  • Concentration
    25 ng/µL

Resource Information

Depositor Comments

In addition to dCas9-KRAB (#46911), another dCas9-containing construct that can be used with these libraries is: pHR-SFFV-KRAB-dCas9-P2A-mCherry (#60954).

The data analysis pipeline for analyzing sequencing data is available on GitHub.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    hCRISPRi-v2 library was a gift from Jonathan Weissman (Addgene ID #)

  • For your References section:

    Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation . Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760.PubMed 27661255