B. subtilis Strain Collection
(Kit #
1000000115, 1000000116
)
Depositing Labs: Carol Gross
Bacillus subtilis is a key Gram positive model bacterium used to study diverse research topics such as chromosome replication, competence, or sporulation. Despite being well-studied and having a fully sequenced genome, there are still many unknown or poorly characterized genes. To facilitate better understanding of individual B. subtilis genes, the Gross lab at UCSF has constructed 2 ordered libraries:
Kit 1000000115
B. subtilis Single Gene Deletion Library--Kanamycin is an arrayed deletion library in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.
This kit will be sent as bacterial glycerol stocks (~100uL/well) in a 384-well plate format.
Kit 1000000116
B. subtilis CRISPRi Essential Gene Knockdown Library is an arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.
This kit will be sent as bacterial glycerol stocks (~100uL/well) in a 384-well plate format.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Kit | 1000000115 | B. subtilis Single Gene Deletion Library--Kanamycin | 1 | $ 5250 | Add to Cart | |
| Kit | 1000000116 | B. subtilis CRISPRi Essential Gene Knockdown Library | 1 | $ 1200 | Add to Cart | |
Description
Publication List for Kits:
- Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis. (Koo BM, et al., 2017)
- Kit #1000000115 - B. subtilis Single Gene Deletion Library--Kanamycin
- A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria. (Peters JM, et al., 2016)
- Kit #1000000116 - B. subtilis CRISPRi Essential Gene Knockdown Library
Kit 1000000115: B. subtilis Single Gene Deletion Library--Kanamycin
A comprehensive single-gene deletion library of the model bacterium B. subtilis 168. The entire protein coding region (not including start and stop codons) for every non-essential gene was replaced with a kanamycin cassette. The library was designed to allow for easy removal of the kan-cassette via Cre recombination and includes barcodes and universal priming sites to enable high-throughput approaches.
The collection includes 3984 knockout strains arrayed in 384-well format (listed in 'Kit Contents' tab or download plate maps under 'Protocols & Resources'). Strains can be grown at 37C. Kanamycin (7.5ug/mL) is recommended for plate selection; 3ug/mL kanamycin can be used for liquid media, but is not necessarily required.
Kit #1000000116: B. subtilis CRISPRi Essential Gene Knockdown Library
A comprehensive arrayed knockdown library targeting all the essential genes from the model bacterium B. subtilis 168. The library was designed using CRISPR interference (CRISPRi) technology and includes a xylose-inducible promoter driving dCas9 paired with a single sgRNA driven by a strong constitutive promoter targeting the 5’ end of each essential gene.
The collection includes 384 CRISPRi strains arrayed in 384-well format (listed in 'Kit Contents tab' or download plate maps under 'Protocols & Resources'). Strains can be grown at 37C under Chloramphenicol selection (6ug/mL).
Resources
These materials are only being distributed by Addgene as full collections. Please contact the Bacillus Genetic Stock Center to obtain individual strains included in Kit 1000000115, Kit 1000000116, or to request strains from the erythromycin-knockout library (not available through Addgene). Please note that Addgene has not sequence-verified these strains and the collections are distributed "as is" based on the depositor's information. We strongly recommend verifying the strains prior to use.
Strain and Plate Data
- BSubtilis Single Gene Deletion Library Data 669.3Â KB
- Bsubtilis CRISPRi Essential Gene KD Data 73.9Â KB
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The B. subtilis Strain Collection was a gift from Carol Gross (Addgene kit #).”
For your Reference section:
Kit #1000000115: B. subtilis Single Gene Deletion Library--Kanamycin
Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis. Koo BM, Kritikos G, Farelli JD, Todor H, Tong K, Kimsey H, Wapinski I, Galardini M, Cabal A, Peters JM, Hachmann AB, Rudner DZ, Allen KN, Typas A, Gross CA. Cell Syst. 2017 Mar 22;4(3):291-305.e7. doi: 10.1016/j.cels.2016.12.013. PubMed PMID: 28189581.
Kit #1000000116: B. subtilis CRISPRi Essential Gene Knockdown Library
A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria. Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CHS, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA. Cell. 2016 Jun 2;165(6):1493-1506. doi: 10.1016/j.cell.2016.05.003. PubMed PMID: 27238023.
B. subtilis Single Gene Deletion Library--Kanamycin - #1000000115
BSubtilis Single Gene Deletion Library Data 669.3Â KBB. subtilis CRISPRi Essential Gene Knockdown Library - #1000000116
| Well Number | Name |
|---|---|
| A01 | Strain name: CAG74399_1; Gene target: no_sgRNA |
| A02 | Strain name: CAG74399_21; Gene target: no_sgRNA |
| A03 | Strain name: CAG74399_2; Gene target: no_sgRNA |
| A04 | Strain name: CAG74399_22; Gene target: no_sgRNA |
| A05 | Strain name: CAG74399_3; Gene target: no_sgRNA |
| A06 | Strain name: CAG74399_23; Gene target: no_sgRNA |
| A07 | Strain name: CAG74399_4; Gene target: no_sgRNA |
| A08 | Strain name: CAG74399_24; Gene target: no_sgRNA |
| A09 | Strain name: CAG74399_5; Gene target: no_sgRNA |
| A10 | Strain name: CAG74399_25; Gene target: no_sgRNA |
| A11 | Strain name: CAG74399_6; Gene target: no_sgRNA |
| A12 | Strain name: CAG74399_26; Gene target: no_sgRNA |
| A13 | Strain name: CAG74399_7; Gene target: no_sgRNA |
| A14 | Strain name: CAG74399_27; Gene target: no_sgRNA |
| A15 | Strain name: CAG74399_8; Gene target: no_sgRNA |
| A16 | Strain name: CAG74399_28; Gene target: no_sgRNA |
| A17 | Strain name: CAG74399_9; Gene target: no_sgRNA |
| A18 | Strain name: CAG74399_29; Gene target: no_sgRNA |
| A19 | Strain name: CAG74399_10; Gene target: no_sgRNA |
| A20 | Strain name: CAG74399_30; Gene target: no_sgRNA |
| A21 | Strain name: CAG74399_11; Gene target: no_sgRNA |
| A22 | Strain name: CAG74399_31; Gene target: no_sgRNA |
| A23 | Strain name: CAG74399_12; Gene target: no_sgRNA |
| A24 | Strain name: CAG74399_32; Gene target: no_sgRNA |
| B01 | Strain name: CAG74399_40; Gene target: no_sgRNA |
| B02 | Strain name: BEC22600; Gene target: aroE |
| B03 | Strain name: BEC22710; Gene target: aroF |
| B04 | Strain name: BEC08930; Gene target: cspR |
| B06 | Strain name: BEC14180; Gene target: dapH |
| B07 | Strain name: BEC22450; Gene target: cca |
| B08 | Strain name: BEC15910; Gene target: fabG |
| B09 | Strain name: BEC15700; Gene target: coaBC |
| B10 | Strain name: BEC27940; Gene target: rpmA |
| B11 | Strain name: BEC25210; Gene target: dnaG |
| B12 | Strain name: BEC10130; Gene target: hemH |
| B13 | Strain name: BEC14190; Gene target: dapL |
| B14 | Strain name: BEC10140; Gene target: hemY |
| B15 | Strain name: BEC00620; Gene target: divIC |
| B16 | Strain name: BEC31650; Gene target: mrpF |
| B17 | Strain name: BEC00010; Gene target: dnaA |
| B18 | Strain name: BEC17390; Gene target: nrdF |
| B19 | Strain name: BEC28990; Gene target: dnaB |
| B20 | Strain name: BEC23130; Gene target: resC |
| B21 | Strain name: BEC29230; Gene target: dnaE |
| B22 | Strain name: BEC15920; Gene target: acpP |
| B23 | Strain name: BEC22750; Gene target: ubiE |
| B24 | Strain name: CAG74399_61; Gene target: no_sgRNA |
| C01 | Strain name: CAG74399_13; Gene target: no_sgRNA |
| C02 | Strain name: BEC28980; Gene target: dnaI |
| C03 | Strain name: BEC06720; Gene target: yerQ |
| C04 | Strain name: BEC01780; Gene target: glmS |
| C05 | Strain name: BEC32710; Gene target: sufC |
| C06 | Strain name: BEC00500; Gene target: glmU |
| C07 | Strain name: BEC31610; Gene target: mrpB |
| C08 | Strain name: BEC25270; Gene target: glyQ |
| C09 | Strain name: BEC15900; Gene target: fabD |
| C10 | Strain name: BEC27410; Gene target: alaS |
| C11 | Strain name: BEC14530; Gene target: rnjA |
| C12 | Strain name: BEC28150; Gene target: hemC |
| C13 | Strain name: BEC16500; Gene target: tsf |
| C14 | Strain name: BEC04560; Gene target: ddl |
| C15 | Strain name: BEC21810; Gene target: dfrA |
| C16 | Strain name: BEC27500; Gene target: mnmA |
| C17 | Strain name: BEC32670; Gene target: sufB |
| C18 | Strain name: BEC29200; Gene target: accA |
| C19 | Strain name: BEC01440; Gene target: rplQ |
| C20 | Strain name: BEC24340; Gene target: accC |
| C21 | Strain name: BEC28080; Gene target: folC |
| C22 | Strain name: BEC01370; Gene target: adk |
| C23 | Strain name: BEC19360; Gene target: odhB |
| C24 | Strain name: CAG74399_33; Gene target: no_sgRNA |
| D01 | Strain name: CAG74399_41; Gene target: no_sgRNA |
| D02 | Strain name: BEC01500; Gene target: rpsI |
| D03 | Strain name: BEC16550; Gene target: dxr |
| D04 | Strain name: BEC01100; Gene target: rpsL |
| D05 | Strain name: BEC24270; Gene target: dxs |
| D06 | Strain name: BEC01410; Gene target: rpsM |
| D07 | Strain name: BEC22840; Gene target: engA |
| D08 | Strain name: BEC01200; Gene target: rpsS |
| D09 | Strain name: BEC33900; Gene target: eno |
| D10 | Strain name: BEC_TRNA_23; Gene target: trnH |
| D11 | Strain name: BEC25290; Gene target: era |
| D12 | Strain name: BEC04620.50.1; Gene target: acpS50_1 |
| D13 | Strain name: BEC11340; Gene target: fabF |
| D14 | Strain name: BEC15240; Gene target: divIB |
| D15 | Strain name: BEC15730; Gene target: fmt |
| D16 | Strain name: BEC22440; Gene target: birA |
| D17 | Strain name: BEC22780; Gene target: folE |
| D18 | Strain name: BEC13300; Gene target: mgtE |
| D19 | Strain name: BEC00790; Gene target: folK |
| D20 | Strain name: BEC27880; Gene target: nifS |
| D21 | Strain name: BEC15150; Gene target: ftsL |
| D22 | Strain name: BEC14580; Gene target: pdhA |
| D23 | Strain name: BEC16530; Gene target: uppS |
| D24 | Strain name: CAG74399_62; Gene target: no_sgRNA |
| E01 | Strain name: CAG74399_14; Gene target: no_sgRNA |
| E02 | Strain name: BEC22760; Gene target: hepS |
| E03 | Strain name: BEC32170; Gene target: dapF |
| E04 | Strain name: BEC25560; Gene target: holA |
| E05 | Strain name: BEC28390; Gene target: racE |
| E06 | Strain name: BEC04640; Gene target: alrA |
| E07 | Strain name: BEC03150; Gene target: aroK |
| E08 | Strain name: BEC09540; Gene target: plsC |
| E09 | Strain name: BEC35720; Gene target: tagF |
| E10 | Strain name: BEC35660; Gene target: mnaA |
| E11 | Strain name: CAG74399_15; Gene target: rpsN |
| E12 | Strain name: BEC27550; Gene target: aspS |
| E13 | Strain name: BEC30790; Gene target: menE |
| E14 | Strain name: BEC16540; Gene target: cdsA |
| E15 | Strain name: BEC31630; Gene target: mrpD |
| E16 | Strain name: BEC22890; Gene target: cmk |
| E17 | Strain name: BEC22490; Gene target: dapB |
| E18 | Strain name: BEC00900; Gene target: ispD |
| E19 | Strain name: BEC06680; Gene target: gatA |
| E20 | Strain name: BEC01380; Gene target: mapA |
| E21 | Strain name: BEC36760; Gene target: murAA |
| E22 | Strain name: BEC00380; Gene target: metS |
| E23 | Strain name: BEC28190; Gene target: engB |
| E24 | Strain name: CAG74399_34; Gene target: no_sgRNA |
| F01 | Strain name: CAG74399_42; Gene target: no_sgRNA |
| F02 | Strain name: BEC28850; Gene target: rplT |
| F03 | Strain name: BEC01770; Gene target: glmM |
| F04 | Strain name: BEC01290; Gene target: rpsNA |
| F05 | Strain name: BEC36900; Gene target: glyA |
| F06 | Strain name: BEC14840.27.2; Gene target: ylaN27_2 |
| F07 | Strain name: BEC15680; Gene target: gmk |
| F08 | Strain name: BEC14840.87; Gene target: ylaN87 |
| F09 | Strain name: BEC22830; Gene target: gpsA |
| F10 | Strain name: BEC14840.189; Gene target: ylaN189 |
| F11 | Strain name: BEC06030; Gene target: groEL |
| F12 | Strain name: BEC14840.192; Gene target: ylaN192 |
| F13 | Strain name: BEC06020; Gene target: groES |
| F14 | Strain name: CAG74399_63; Gene target: no_sgRNA |
| F15 | Strain name: BEC00090; Gene target: guaB |
| F16 | Strain name: BEC12519; Gene target: yjzJ |
| F17 | Strain name: BEC00070; Gene target: gyrA |
| F18 | Strain name: BEC23230; Gene target: ypuF |
| F19 | Strain name: BEC28170; Gene target: hemA |
| F20 | Strain name: CAG74399_64; Gene target: no_sgRNA |
| F21 | Strain name: BEC12230; Gene target: yjjA |
| F22 | Strain name: BEC09510; Gene target: yhdL |
| F23 | Strain name: BEC10120; Gene target: hemE |
| F24 | Strain name: CAG74399_65; Gene target: no_sgRNA |
| G01 | Strain name: CAG74399_16; Gene target: no_sgRNA |
| G02 | Strain name: BEC28030; Gene target: mreB |
| G03 | Strain name: BEC15890; Gene target: plsX |
| G04 | Strain name: BEC28020; Gene target: mreC |
| G05 | Strain name: BEC00310; Gene target: holB |
| G06 | Strain name: BEC28010; Gene target: mreD |
| G07 | Strain name: BEC18100; Gene target: parC |
| G08 | Strain name: BEC15200; Gene target: murD |
| G09 | Strain name: BEC16510; Gene target: pyrH |
| G10 | Strain name: BEC35750; Gene target: tagA |
| G11 | Strain name: BEC28640; Gene target: pheS |
| G12 | Strain name: BEC17370; Gene target: nrdI |
| G13 | Strain name: BEC29060; Gene target: coaE |
| G14 | Strain name: BEC22420; Gene target: panC |
| G15 | Strain name: BEC01130; Gene target: tufA |
| G16 | Strain name: BEC06070; Gene target: ydiP |
| G17 | Strain name: BEC27960; Gene target: rplU |
| G18 | Strain name: BEC15160; Gene target: pbpB |
| G19 | Strain name: BEC18070; Gene target: yneS |
| G20 | Strain name: BEC00190; Gene target: dnaX |
| G21 | Strain name: BEC27560; Gene target: hisS |
| G22 | Strain name: BEC16920; Gene target: pgsA |
| G23 | Strain name: BEC35530; Gene target: tagO |
| G24 | Strain name: CAG74399_35; Gene target: no_sgRNA |
| H01 | Strain name: CAG74399_43; Gene target: no_sgRNA |
| H02 | Strain name: BEC07840; Gene target: yfkN |
| H03 | Strain name: BEC15430; Gene target: ileS |
| H04 | Strain name: BEC16030; Gene target: trmD |
| H05 | Strain name: BEC28870; Gene target: infC |
| H06 | Strain name: BEC16760; Gene target: dapG |
| H07 | Strain name: BEC00460; Gene target: ipk |
| H08 | Strain name: BEC14850; Gene target: ftsW |
| H09 | Strain name: BEC00910; Gene target: ispF |
| H10 | Strain name: CAG74399_66; Gene target: ftsZ |
| H11 | Strain name: BEC25070; Gene target: ispG |
| H12 | Strain name: BEC00920; Gene target: gltX |
| H13 | Strain name: BEC25160; Gene target: ispH |
| H14 | Strain name: BEC00060; Gene target: gyrB |
| H15 | Strain name: BEC30320; Gene target: leuS |
| H16 | Strain name: BEC00820; Gene target: lysS |
| H17 | Strain name: BEC21320; Gene target: yomL |
| H18 | Strain name: BEC30770; Gene target: mntA |
| H19 | Strain name: BEC38490; Gene target: menA |
| H20 | Strain name: BEC15190; Gene target: mraY |
| H21 | Strain name: BEC30780; Gene target: menC |
| H22 | Strain name: BEC15180; Gene target: murE |
| H23 | Strain name: BEC30820; Gene target: menD |
| H24 | Strain name: CAG74399_67; Gene target: no_sgRNA |
| I01 | Strain name: CAG74399_17; Gene target: no_sgRNA |
| I02 | Strain name: BEC35290; Gene target: prfB |
| I03 | Strain name: BEC15220; Gene target: murG |
| I04 | Strain name: BEC37150; Gene target: pyrG |
| I05 | Strain name: BEC00020; Gene target: dnaN |
| I06 | Strain name: BEC41050; Gene target: rnpA |
| I07 | Strain name: BEC05940; Gene target: gcp |
| I08 | Strain name: BEC_MISC_RNA_35; Gene target: rnpB |
| I09 | Strain name: BEC29210; Gene target: accD |
| I10 | Strain name: BEC01190; Gene target: rplB |
| I11 | Strain name: BEC01320.2; Gene target: rplR-2 |
| I12 | Strain name: BEC01050; Gene target: rplL |
| I13 | Strain name: BEC01330; Gene target: rpsE |
| I14 | Strain name: BEC01390; Gene target: infA |
| I15 | Strain name: BEC00770; Gene target: sul |
| I16 | Strain name: BEC16630; Gene target: infB |
| I17 | Strain name: BEC29670; Gene target: tyrS |
| I18 | Strain name: BEC32680; Gene target: iscU |
| I19 | Strain name: BEC23840; Gene target: yqjK |
| I20 | Strain name: BEC06620; Gene target: ligA |
| I21 | Strain name: BEC25260; Gene target: glyS |
| I22 | Strain name: BEC06670; Gene target: gatC |
| I23 | Strain name: BEC00940; Gene target: cysS |
| I24 | Strain name: CAG74399_36; Gene target: no_sgRNA |
| J01 | Strain name: CAG74399_44; Gene target: no_sgRNA |
| J02 | Strain name: BEC03130; Gene target: nadE |
| J03 | Strain name: BEC40400; Gene target: walK |
| J04 | Strain name: BEC17380; Gene target: nrdE |
| J05 | Strain name: CAG74399_45; Gene target: yqaE |
| J06 | Strain name: BEC16600; Gene target: nusA |
| J07 | Strain name: BEC12520; Gene target: xkdB |
| J08 | Strain name: BEC16580; Gene target: polC |
| J09 | Strain name: BEC31600; Gene target: mrpA |
| J10 | Strain name: BEC11610; Gene target: ppnKB |
| J11 | Strain name: BEC31660; Gene target: mrpG |
| J12 | Strain name: BEC37010; Gene target: prfA |
| J13 | Strain name: BEC15230; Gene target: murB |
| J14 | Strain name: BEC38120; Gene target: rodA |
| J15 | Strain name: BEC29790; Gene target: murC |
| J16 | Strain name: BEC00130; Gene target: serS |
| J17 | Strain name: BEC32110; Gene target: yumC |
| J18 | Strain name: BEC14840.27.1; Gene target: ylaN27_1 |
| J19 | Strain name: BEC38690; Gene target: yxlC |
| J20 | Strain name: BEC15290.226; Gene target: ftsZ226 |
| J21 | Strain name: BEC06610; Gene target: pcrA |
| J22 | Strain name: BEC09950; Gene target: prsA |
| J23 | Strain name: BEC33910; Gene target: pgm |
| J24 | Strain name: CAG74399_68; Gene target: no_sgRNA |
| K01 | Strain name: CAG74399_18; Gene target: no_sgRNA |
| K02 | Strain name: BEC31640; Gene target: mrpE |
| K03 | Strain name: BEC01340; Gene target: rpmD |
| K04 | Strain name: BEC25640; Gene target: nadD |
| K05 | Strain name: BEC35710; Gene target: tagG |
| K06 | Strain name: BEC14000; Gene target: patA |
| K07 | Strain name: BEC28130; Gene target: hemB |
| K08 | Strain name: BEC40550; Gene target: ppaC |
| K09 | Strain name: BEC16770; Gene target: dapA |
| K10 | Strain name: BEC01230; Gene target: rplP |
| K11 | Strain name: BEC23220; Gene target: scpA |
| K12 | Strain name: BEC00680; Gene target: hprT |
| K13 | Strain name: BEC06690; Gene target: gatB |
| K14 | Strain name: BEC29660; Gene target: rpsD |
| K15 | Strain name: BEC16520; Gene target: frr |
| K16 | Strain name: BEC01250; Gene target: rpsQ |
| K17 | Strain name: BEC22790; Gene target: hbs |
| K18 | Strain name: BEC40890; Gene target: rpsR |
| K19 | Strain name: BEC32690; Gene target: sufS |
| K20 | Strain name: BEC35300; Gene target: secA |
| K21 | Strain name: BEC31620; Gene target: mrpC |
| K22 | Strain name: BEC21490; Gene target: sunI |
| K23 | Strain name: BEC01120; Gene target: fusA |
| K24 | Strain name: CAG74399_37; Gene target: no_sgRNA |
| L01 | Strain name: CAG74399_46; Gene target: no_sgRNA |
| L02 | Strain name: BEC01320.105; Gene target: rplR105 |
| L03 | Strain name: BEC05920; Gene target: ydiC |
| L04 | Strain name: BEC04620.50.2; Gene target: acpS50_2 |
| L05 | Strain name: BEC06060; Gene target: ydiO |
| L06 | Strain name: BEC26350.101; Gene target: yqaE101 |
| L07 | Strain name: BEC00510; Gene target: prs |
| L08 | Strain name: BEC06811.130; Gene target: yezG130 |
| L09 | Strain name: BEC16050; Gene target: rbgA |
| L10 | Strain name: BEC24330; Gene target: yqhY |
| L11 | Strain name: BEC23140; Gene target: resB |
| L12 | Strain name: BEC03100; Gene target: ycgG |
| L13 | Strain name: BEC16670; Gene target: ribC |
| L14 | Strain name: BEC24310; Gene target: folD |
| L15 | Strain name: CAG74399_47; Gene target: yezG |
| L16 | Strain name: BEC15280; Gene target: ftsA |
| L17 | Strain name: BEC01160; Gene target: rplC |
| L18 | Strain name: BEC30800; Gene target: menB |
| L19 | Strain name: BEC01170; Gene target: rplD |
| L20 | Strain name: BEC22740; Gene target: hepT |
| L21 | Strain name: BEC01280; Gene target: rplE |
| L22 | Strain name: BEC16750; Gene target: asd |
| L23 | Strain name: BEC01310; Gene target: rplF |
| L24 | Strain name: CAG74399_69; Gene target: no_sgRNA |
| M01 | Strain name: CAG74399_19; Gene target: no_sgRNA |
| M02 | Strain name: BEC01270; Gene target: rplX |
| M03 | Strain name: BEC15980; Gene target: ffh |
| M04 | Strain name: BEC01080; Gene target: rpoC |
| M05 | Strain name: BEC24350; Gene target: accB |
| M06 | Strain name: BEC01150; Gene target: rpsJ |
| M07 | Strain name: BEC33940; Gene target: gapA |
| M08 | Strain name: BEC01420; Gene target: rpsK |
| M09 | Strain name: BEC01000; Gene target: secE |
| M10 | Strain name: BEC30550; Gene target: metK |
| M11 | Strain name: BEC00780; Gene target: folB |
| M12 | Strain name: BEC32700; Gene target: sufD |
| M13 | Strain name: BEC16490; Gene target: rpsB |
| M14 | Strain name: BEC35760; Gene target: tagB |
| M15 | Strain name: BEC18090; Gene target: parE |
| M16 | Strain name: BEC34790; Gene target: trxB |
| M17 | Strain name: BEC40440; Gene target: dnaC |
| M18 | Strain name: BEC28090; Gene target: valS |
| M19 | Strain name: BEC22350; Gene target: dnaD |
| M20 | Strain name: BEC12530; Gene target: xkdC |
| M21 | Strain name: BEC36410; Gene target: mbl |
| M22 | Strain name: BEC05100; Gene target: yddT |
| M23 | Strain name: BEC28500; Gene target: trxA |
| M24 | Strain name: CAG74399_38; Gene target: no_sgRNA |
| N01 | Strain name: CAG74399_48; Gene target: no_sgRNA |
| N02 | Strain name: BEC29050; Gene target: ytbE |
| N03 | Strain name: BEC01490; Gene target: rplM |
| N04 | Strain name: BEC15020; Gene target: coaD |
| N05 | Strain name: BEC16040; Gene target: rplS |
| N06 | Strain name: BEC11420; Gene target: trpS |
| N07 | Strain name: BEC01070; Gene target: rpoB |
| N08 | Strain name: BEC01300; Gene target: rpsH |
| N09 | Strain name: BEC01110; Gene target: rpsG |
| N10 | Strain name: CAG74399_70; Gene target: no_sgRNA |
| N11 | Strain name: BEC16680; Gene target: rpsO |
| N12 | Strain name: CAG74399_71; Gene target: no_sgRNA |
| N13 | Strain name: BEC15990; Gene target: rpsP |
| N14 | Strain name: CAG74399_72; Gene target: no_sgRNA |
| N15 | Strain name: BEC_MISC_RNA_2; Gene target: scr |
| N16 | Strain name: CAG74399_73; Gene target: no_sgRNA |
| N17 | Strain name: BEC25200; Gene target: sigA |
| N18 | Strain name: CAG74399_74; Gene target: no_sgRNA |
| N19 | Strain name: BEC15940; Gene target: smc |
| N20 | Strain name: CAG74399_75; Gene target: no_sgRNA |
| N21 | Strain name: BEC35700; Gene target: tagH |
| N22 | Strain name: CAG74399_76; Gene target: no_sgRNA |
| N23 | Strain name: BEC00670; Gene target: tilS |
| N24 | Strain name: CAG74399_77; Gene target: no_sgRNA |
| O01 | Strain name: CAG74399_20; Gene target: no_sgRNA |
| O02 | Strain name: BEC01040; Gene target: rplJ |
| O03 | Strain name: BEC15930; Gene target: rnc |
| O04 | Strain name: BEC16960; Gene target: ymdA |
| O05 | Strain name: BEC15830; Gene target: yloU |
| O06 | Strain name: BEC17910; Gene target: yneF |
| O07 | Strain name: BEC15710; Gene target: priA |
| O08 | Strain name: BEC25670; Gene target: yqeH |
| O09 | Strain name: BEC28630; Gene target: pheT |
| O10 | Strain name: BEC28650; Gene target: ysgA |
| O11 | Strain name: BEC04570; Gene target: murF |
| O12 | Strain name: BEC35740; Gene target: tagD |
| O13 | Strain name: BEC01260; Gene target: rplN |
| O14 | Strain name: BEC01430; Gene target: rpoA |
| O15 | Strain name: BEC40410; Gene target: walR |
| O16 | Strain name: BEC01220; Gene target: rpsC |
| O17 | Strain name: BEC10660; Gene target: yisB |
| O18 | Strain name: BEC35600; Gene target: tuaB |
| O19 | Strain name: BEC16570; Gene target: proS |
| O20 | Strain name: BEC01360; Gene target: secY |
| O21 | Strain name: BEC33660; Gene target: rghRA |
| O22 | Strain name: BEC40900; Gene target: ssbA |
| O23 | Strain name: BEC00530; Gene target: pth |
| O24 | Strain name: CAG74399_39; Gene target: no_sgRNA |
| P01 | Strain name: CAG74399_49; Gene target: no_sgRNA |
| P02 | Strain name: CAG74399_78; Gene target: no_sgRNA |
| P03 | Strain name: CAG74399_50; Gene target: no_sgRNA |
| P04 | Strain name: CAG74399_79; Gene target: no_sgRNA |
| P05 | Strain name: CAG74399_51; Gene target: no_sgRNA |
| P06 | Strain name: CAG74399_80; Gene target: no_sgRNA |
| P07 | Strain name: CAG74399_52; Gene target: no_sgRNA |
| P08 | Strain name: CAG74399_81; Gene target: no_sgRNA |
| P09 | Strain name: CAG74399_53; Gene target: no_sgRNA |
| P10 | Strain name: CAG74399_82; Gene target: no_sgRNA |
| P11 | Strain name: CAG74399_54; Gene target: no_sgRNA |
| P12 | Strain name: CAG74399_83; Gene target: no_sgRNA |
| P13 | Strain name: CAG74399_55; Gene target: no_sgRNA |
| P14 | Strain name: CAG74399_84; Gene target: no_sgRNA |
| P15 | Strain name: CAG74399_56; Gene target: no_sgRNA |
| P16 | Strain name: CAG74399_85; Gene target: no_sgRNA |
| P17 | Strain name: CAG74399_57; Gene target: no_sgRNA |
| P18 | Strain name: CAG74399_86; Gene target: no_sgRNA |
| P19 | Strain name: CAG74399_58; Gene target: no_sgRNA |
| P20 | Strain name: CAG74399_87; Gene target: no_sgRNA |
| P21 | Strain name: CAG74399_59; Gene target: no_sgRNA |
| P22 | Strain name: CAG74399_88; Gene target: no_sgRNA |
| P23 | Strain name: CAG74399_60; Gene target: no_sgRNA |
| P24 | Strain name: CAG74399_89; Gene target: no_sgRNA |
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