B52 (empty plasmid backbone to express 2 sgRNAs)
(Plasmid #100708)

• Purpose: (Empty Backbone) Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)
• Depositing Lab: Alberto Ciccia
• Publication: Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 10.1016/j.molcel.2017.08.008.

Backbone

• Vector backbone: B52 (MLM3636 plasmid backbone modified to express 2 sgRNAs)
• Backbone size (bp): 2633
• Modifications to backbone: The B52 plasmid is a derivative of the MLM3636 construct (Addgene #43860). B52 contains a second sgRNA-expressing cassette, which was PCR amplified from the plasmid eSpCas9 (1.1) (Addgene #71814) and cloned into the MLM3636 plasmid using the restriction enzymes HindIII and XhoI. The final B52 plasmid contains a ColE1 origin, an ampicillin resistance gene and two sgRNA expression cassettes with either BsmBI or BbsI sites for sgRNA cloning.
• Vector type: Mammalian Expression, CRISPR
• Promoter: U6 promoters

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry