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FRB-mCherry-FTH1
(Plasmid #100749)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100749 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherry-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ferritin heavy chain 1
  • Alt name
    Ferritin heavy chain
  • Species
    H. sapiens (human)
  • Entrez Gene
    FTH1 (a.k.a. FHC, FTH, FTHL6, HFE5, PIG15, PLIF)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FRB (N terminal on insert)
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRII (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRB-mCherry-FTH1 was a gift from Stephen Royle (Addgene plasmid # 100749)
  • For your References section:

    FerriTag is a new genetically-encoded inducible tag for correlative light-electron microscopy. Clarke NI, Royle SJ. Nat Commun. 2018 Jul 4;9(1):2604. doi: 10.1038/s41467-018-04993-0. 10.1038/s41467-018-04993-0 PubMed 29973588