HIP1R-GFP-FKBP
(Plasmid #100752)

• Purpose: Expression of HIP1R with a GFP-FKBP tag in mammalian cells.
• Depositing Lab: Stephen Royle
• Publication: Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 10.1038/s41467-018-04993-0.

Backbone

• Vector backbone: pEGFP-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HIP1R
• Alt name: KIAA0655
• Species: H. sapiens (human)
• Entrez Gene: HIP1R (a.k.a. HIP12, HIP3, ILWEQ)
• Promoter: CMV
• Tags / Fusion Proteins:
  • GFP (C terminal on insert)
  • FKBP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Supplemental Documents:
  • HIP1R-GFP-FKBP.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry