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LLP339 pGK-dCas9-SunTag
(Plasmid #100943)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100943 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    LLP249 pGK-dCas9-DNMT3a V3
  • Backbone size w/o insert (bp) 5675
  • Total vector size (bp) 10815
  • Modifications to backbone
    Puro driven by pEF1a
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9, SunTag array (10xGCN4)
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
    1596
  • Mutation
    D10A, H840A for dCas9
  • Tag / Fusion Protein
    • 3xHA (N terminal on insert)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LLP339 pGK-dCas9-SunTag was a gift from Ryan Lister (Addgene plasmid # 100943 ; http://n2t.net/addgene:100943 ; RRID:Addgene_100943)
  • For your References section:

    A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs. Pflueger C, Tan D, Swain T, Nguyen TV, Pflueger J, Nefzger C, Polo JM, Ford E, Lister R. Genome Res. 2018 Jun 15. pii: gr.233049.117. doi: 10.1101/gr.233049.117. 10.1101/gr.233049.117 PubMed 29907613