pMflT-o4
(Plasmid
#101312)
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PurposeContains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101312 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonecolE1
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
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Alt nameComplete oriC region of M. florum L1
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SpeciesMesoplasma florum
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMflT-o4 was a gift from Sébastien Rodrigue (Addgene plasmid # 101312 ; http://n2t.net/addgene:101312 ; RRID:Addgene_101312) -
For your References section:
Development of oriC-Based Plasmids for Mesoplasma florum. Matteau D, Pepin ME, Baby V, Gauthier S, Arango Giraldo M, Knight TF, Rodrigue S. Appl Environ Microbiol. 2017 Mar 17;83(7). pii: e03374-16. doi: 10.1128/AEM.03374-16. Print 2017 Apr 1. 10.1128/AEM.03374-16 PubMed 28115382
Map uploaded by the depositor.
