pCXN2-FLAG-AT2R-CFP
(Plasmid #101660)

• Purpose: Expresses AT2R with FLAG tag and CFP in mammalian cells.
• Depositing Lab: Yusuke Ohba
• Publication: Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.1038/srep21613.

Backbone

• Vector backbone: pCXN2
• Total vector size (bp): 7840
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha is also a suitable growth strain.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Angiotensin II type 2 receptor
• Alt name: AT2R
• Alt name: AGTR2
• Species: H. sapiens (human)
• Insert Size (bp): 1124
• GenBank ID: NM_000686.4
• Entrez Gene: AGTR2 (a.k.a. AT2, ATGR2, MRX88)
• Promoter: CAG promoter
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • ECFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (with FLAG tag) (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry