pCB1532H-RFP
(Plasmid #101855)

• Purpose: (Empty Backbone) Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)
• Depositing Lab: Sophien Kamoun
• Publication: Pennington et al Figshare (2017)

Backbone

• Vector backbone: pCB1532
• Backbone manufacturer: Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html
• Backbone size (bp): 4835
• Modifications to backbone: Removal of BsaI sites to domesticate backbone for Golden Gate (GG) cloning. Addition of an RFP marker for red-white selection in E. coli. The RFP is flanked by two BsaI sites. GG reaction leads to the loss of the RFP when the gene is inserted. The RFP is expressed in E. coli, allowing for red-white selection of transformants. Antibiotic resistance changed to Ampicillin
• Vector type: Fungal expression plasmid, tested in Magnaporthe
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• 5′ sequencing primer: GTAAAACGACGGCCAG
• 3′ sequencing primer: CAGGAAACAGCTATGAC

Resource Information

• Supplemental Documents:
  • pCB1532H-RFP.gb
• A portion of this plasmid was derived from a plasmid made by: Plasmid designed by Helen G. Pennington, and cloning performed by Mark Youles (TSL Synbio)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid derived from the pCB1532 vector series Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html