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WPXL LEFTY2 gRNA
(Plasmid #101924)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101924 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    WPLX destination vector
  • Backbone size w/o insert (bp) 12806
  • Total vector size (bp) 11630
  • Modifications to backbone
    removed Puromycin selection and inserted Hygromycin
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LEFTY2 enhancer targeting gRNA
  • gRNA/shRNA sequence
    GAGAAGCAGAGCAGGAAGTC
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer TCTCGACGGTATCGGTTAACTT
  • 3′ sequencing primer TACGGGAAGCAATAGCATGA
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WPXL LEFTY2 gRNA was a gift from Danwei Huangfu (Addgene plasmid # 101924 ; http://n2t.net/addgene:101924 ; RRID:Addgene_101924)
  • For your References section:

    TET proteins safeguard bivalent promoters from de novo methylation in human embryonic stem cells. Verma N, Pan H, Dore LC, Shukla A, Li QV, Pelham-Webb B, Teijeiro V, Gonzalez F, Krivtsov A, Chang CJ, Papapetrou EP, He C, Elemento O, Huangfu D. Nat Genet. 2018 Jan;50(1):83-95. doi: 10.1038/s41588-017-0002-y. Epub 2017 Dec 4. 10.1038/s41588-017-0002-y PubMed 29203910