Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Bacterial strain #102265)

No maps are available for this item.

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Bacterial Strain 102265 Bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Low salt LB (5 g/L)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Requires low salt (5 g/L NaCl or less) in LB
  • Copy number

Resource Information

  • Terms and Licenses

Depositor Comments

Genotype = ΔompC ΔompF
Precursor strain = BL21(DE3) [genotype F– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS)]

Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BL21ΔCF was a gift from Jack Leo (Addgene plasmid # 102265)
  • For your References section:

    A New Strain Collection for Improved Expression of Outer Membrane Proteins. Meuskens I, Michalik M, Chauhan N, Linke D, Leo JC. Front Cell Infect Microbiol. 2017 Nov 7;7:464. doi: 10.3389/fcimb.2017.00464. eCollection 2017. 10.3389/fcimb.2017.00464 PubMed 29164072