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LbCpf1-2NLS
(Plasmid #102566)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102566 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LbCpf1
  • Species
    Synthetic
  • Insert Size (bp)
    5000
  • Promoter T7
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Cloning Information

Resource Information

Depositor Comments

"CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing" - Moreno-Mateos et al.
https://www.biorxiv.org/content/early/2017/06/26/156125

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LbCpf1-2NLS was a gift from Jennifer Doudna (Addgene plasmid # 102566 ; http://n2t.net/addgene:102566 ; RRID:Addgene_102566)
  • For your References section:

    CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. Moreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E, Khokha MK, Doudna JA, Giraldez AJ. Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2. 10.1038/s41467-017-01836-2 [pii] PubMed 29222508