pUDC175
(Plasmid #103019)

• Purpose: S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter
• Depositing Lab: Jean-Marc Daran
• Publication: Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):12585-12598. doi: 10.1093/nar/gkx1007.

Backbone

• Vector backbone: p414TEF1
• Backbone manufacturer: Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 15
• Backbone size w/o insert (bp): 5416
• Total vector size (bp): 9466
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fncpf1
• Species: Francisella tularensis subsp. novicida U112
• Insert Size (bp): 4038
• Mutation: human codon optimized
• GenBank ID: CP000439.1
• Promoter: TEF1
• Tags / Fusion Proteins:
  • NLS (C terminal on insert)
  • 3HA (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTATGAGCATCTACCAGGAGTT
• 3′ sequencing primer: TCCTTTTCGGTTAGAGCGGATGTGGGGGGAGGGCGTGAATGTAAGCGTGACATAACTAATTTAGGCATAGTCGGGGACAT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Zetsche et al., 2015, Cell 163, 759–771. http://dx.doi.org/10.1016/j.cell.2015.09.038
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry