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pmCherry-MS2coatProtein
(Plasmid #103832)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 103832 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3941
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-MS2 coat protein
  • Insert Size (bp)
    1102
  • Mutation
    MCP: nt 202-240 are missing (13 aa), C242G (A81G), A246C (silent, A82A), T291C (silent, A97A)
  • Entrez Gene
    cp (a.k.a. MS2g2)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EGFP was cut out from the backbone (AgeI/BamHI cuts); relevance of mutations unknown, but insert sequence is almost identical (except A88G, I30V) to another published MCP sequence (GenBank: JQ624676.1, Auslaender et al. 2012, Nature 487(7405): 123-127)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry-MS2coatProtein was a gift from Karsten Rippe (Addgene plasmid # 103832 ; http://n2t.net/addgene:103832 ; RRID:Addgene_103832)
  • For your References section:

    Real-time observation of light-controlled transcription in living cells. Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K. J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. Epub 2017 Nov 9. 10.1242/jcs.205534 PubMed 29122982