This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed December 24th & 25th and December 31st & January 1st. For details, see our holiday shipping schedule. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pEB2-mRuby3 Addgene
(Plasmid #104005)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104005 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEB2
  • Backbone size w/o insert (bp) 3224
  • Total vector size (bp) 3935
  • Modifications to backbone
    See "Notes On Plasmids In This Collection"
  • Vector type
    Bacterial Expression ; Low copy

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MG1655
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mRuby3 Addgene
  • Species
    Synthetic
  • Insert Size (bp)
    711
  • Mutation
    Mutations relative to eqFP611 (MSKGEE LIKENMRMKVVMEG… M12K, Y22H, D31E, N33R, M36E, T38V, V46I, K67R, H72Y, T73P, K74A, G75D, F102V, M105T, C114E, H118N, A119V, T122R, A131P, L147M, S158T, Q159D, M160I, N163K, Y169H, S171H, S173N, E175V, E185G, F187I, F192V, F194A, L202I, K207N, M209T, F210Y, H214R, H216V, F221Y, C222S, D223N … AVAKYSNLGG GMDELYK)
  • Promoter proC

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GCGTATCACGAGGCCCTTTC
  • 3′ sequencing primer AAAGGGAAAACTGTCCATATGCAC
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This FP derives from eqFP611. Please see "Notes On Plasmids In This Collection" document for further details and references.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEB2-mRuby3 Addgene was a gift from Philippe Cluzel (Addgene plasmid # 104005 ; http://n2t.net/addgene:104005 ; RRID:Addgene_104005)
  • For your References section:

    Systematic characterization of maturation time of fluorescent proteins in living cells. Balleza E, Kim JM, Cluzel P. Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 2017 Nov 20. 10.1038/nmeth.4509 PubMed 29320486