PurposeAn AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||104054||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6023
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
Insert Size (bp)1492
- Promoter MBP
/ Fusion Proteins
- NLS (N terminal on insert)
- NLS (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI/Acc56I (not destroyed)
- 3′ cloning site EcoRI/EcoRV (not destroyed)
Please visit https://www.biorxiv.org/content/early/2018/01/19/246405 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-MBP-2xNLS-tdTomato was a gift from Viviana Gradinaru (Addgene plasmid # 104054 ; http://n2t.net/addgene:104054 ; RRID:Addgene_104054)
For your References section:Systemic AAV vectors for widespread and targeted gene delivery in rodents. Challis RC, Ravindra Kumar S, Chan KY, Challis C, Beadle K, Jang MJ, Kim HM, Rajendran PS, Tompkins JD, Shivkumar K, Deverman BE, Gradinaru V. Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3. 10.1038/s41596-018-0097-3 PubMed 30626963