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pGEX2TK-YAP-WW1
(Plasmid #104305)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104305 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-2TK
  • Backbone manufacturer
    Pharmacia
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    YAP WW1 domain
  • Species
    H. sapiens (human)
  • Mutation
    WW domain 1
  • Entrez Gene
    YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The human WW domain in this plasmid was obtained from cDNA described in the following reference:

Sudol M et al. J Biol Chem. 1995 Jun 16;270(24):14733-41. https://www.ncbi.nlm.nih.gov/pubmed/7782338

Please see the following references for additional information regarding the WW domain of human YAP:

1. Chen HI and Sudol M. Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7819-23. https://www.ncbi.nlm.nih.gov/pubmed/7644498

2. Toepert F et al., Angew Chem Int Ed Engl. 2001 Mar 2;40(5):897-900. https://www.ncbi.nlm.nih.gov/pubmed/11241639

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX2TK-YAP-WW1 was a gift from Marius Sudol (Addgene plasmid # 104305 ; http://n2t.net/addgene:104305 ; RRID:Addgene_104305)