pLenti6.3/TO/CD9-GFP
(Plasmid #104402)

• Purpose: Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal
• Depositing Lab: Jens Gruber
• Publication: Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi: 10.1016/j.ymthe.2017.11.008. Epub 2017 Nov 16.

Backbone

• Vector backbone: pLenti6.3/TO/V5-DEST
• Backbone manufacturer: Thermo Fisher
• Backbone size w/o insert (bp): 9351
• Total vector size (bp): 9227
• Modifications to backbone: Gateway inserted
• Vector type: Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CD9
• Alt name: BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29
• Species: H. sapiens (human)
• Insert Size (bp): 1431
• GenBank ID: NM_001769
• Entrez Gene: CD9 (a.k.a. BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEmerald (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: ATTB1

Resource Information

• Supplemental Documents:
  • #118 pLenti CD9 GFP.gb
• A portion of this plasmid was derived from a plasmid made by: Plasmid addgene #54029
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid is used and named CD9-GFP in publication even though it contains mEmerald. Described in the methods of Boker et al 2018 (https://www.ncbi.nlm.nih.gov/pubmed/29221804). Please see publication for more information.

Also see the following article for additional information on using this plasmid: Schiller et al., 2018 https://www.sciencedirect.com/science/article/pii/S2329050118300378