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pHACK-GAL4>QF2(newHA1)
(Plasmid #104873)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104873 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHACK
  • Backbone manufacturer
    Potter Lab
  • Backbone size w/o insert (bp) 4967
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T2A-QF2
  • Alt name
    gRNA1 against GAL4
  • Alt name
    gRNA2 against GAL4
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Insert Size (bp)
    5194

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Depositor Comments

This is an updated version of pHACK-GAL4>QF2 (Addgene#80275) which introduces AvrII/SphI restriction sites to easily excise QF2-hsp70 for cloning of other gene cassettes into a GAL4 HACK construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHACK-GAL4>QF2(newHA1) was a gift from Christopher Potter (Addgene plasmid # 104873 ; http://n2t.net/addgene:104873 ; RRID:Addgene_104873)
  • For your References section:

    Editing Transgenic DNA Components by Inducible Gene Replacement in Drosophila melanogaster. Lin CC, Potter CJ. Genetics. 2016 Jun 22. pii: genetics.116.191783. 10.1534/genetics.116.191783 PubMed 27334272