pUASp-FMW-attB-AthHen1
(Plasmid
#104957)
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PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila germline tissues using Gal4 drivers
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104957 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUASp-FMW
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Backbone manufacturerThermofisher
- Backbone size w/o insert (bp) 12028
- Total vector size (bp) 12969
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Modifications to backbonegateway cloning using pENTR-D-AthHEN1DmeCO (entry vector) and pUASp-FMW-attB (destination vector)
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHen1
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)2829
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Entrez GeneHEN1 (a.k.a. AT4G20910, CORYMBOSA 2, CRM2, HUA ENHANCER 1, T13K14.70, T13K14_70)
- Promoter pTrasposase promoter
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Tag
/ Fusion Protein
- FLAG Myc (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pTransp (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUASp-FMW-attB-AthHen1 was a gift from Stefan Ameres (Addgene plasmid # 104957 ; http://n2t.net/addgene:104957 ; RRID:Addgene_104957) -
For your References section:
Cell-type specific sequencing of microRNAs from complex animal tissues. Alberti C, Manzenreither RA, Sowemimo I, Burkard TR, Wang J, Mahofsky K, Ameres SL, Cochella L. Nat Methods. 2018 Feb 26. pii: nmeth.4610. doi: 10.1038/nmeth.4610. 10.1038/nmeth.4610 PubMed 29481550
Map uploaded by the depositor.
