Purposemitochondrial calcium sensor
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||105009||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG mito-GCaMP5G was a gift from Franck Polleux (Addgene plasmid # 105009 ; http://n2t.net/addgene:105009 ; RRID:Addgene_105009)
For your References section:LKB1 Regulates Mitochondria-Dependent Presynaptic Calcium Clearance and Neurotransmitter Release Properties at Excitatory Synapses along Cortical Axons. Kwon SK, Sando R 3rd, Lewis TL, Hirabayashi Y, Maximov A, Polleux F. PLoS Biol. 2016 Jul 18;14(7):e1002516. doi: 10.1371/journal.pbio.1002516. eCollection 2016 Jul. PBIOLOGY-D-16-00343 [pii] PubMed 27429220