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PurposeTo visualize nuclear dynamics in living cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepERFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRac1
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Alt nameRAC1
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SpeciesH. sapiens (human)
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Mutationaa encoding 172-192
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Entrez GeneRAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
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Tag
/ Fusion Protein
- RFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (unknown if destroyed)
- 3′ cloning site BamH1 (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Human Rac1 sequence spanning nucleotides aa 171-192. The insert was from Dr. Natalie Lamarche-Vane (McGill University).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRFP-NLS was a gift from Kenneth Yamada (Addgene plasmid # 105296 ; http://n2t.net/addgene:105296 ; RRID:Addgene_105296) -
For your References section:
Generation of compartmentalized pressure by a nuclear piston governs cell motility in a 3D matrix. Petrie RJ, Koo H, Yamada KM. Science. 2014 Aug 29;345(6200):1062-5. doi: 10.1126/science.1256965. 10.1126/science.1256965 PubMed 25170155
