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(Plasmid #105296)


Item Catalog # Description Quantity Price (USD)
Plasmid 105296 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Mutation
    aa encoding 172-192
  • Entrez Gene
    RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
  • Tag / Fusion Protein
    • RFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)

Resource Information

Depositor Comments

Human Rac1 sequence spanning nucleotides aa 171-192. The insert was from Dr. Natalie Lamarche-Vane (McGill University).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRFP-NLS was a gift from Kenneth Yamada (Addgene plasmid # 105296 ; ; RRID:Addgene_105296)
  • For your References section:

    Generation of compartmentalized pressure by a nuclear piston governs cell motility in a 3D matrix. Petrie RJ, Koo H, Yamada KM. Science. 2014 Aug 29;345(6200):1062-5. doi: 10.1126/science.1256965. 10.1126/science.1256965 PubMed 25170155