|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||105360||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepICH41295 (Engler et al., 2014)
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namepromotor AP1 (At1g69120;1400bp)
SpeciesA. thaliana (mustard weed)
MutationBsaI/ BpiI restriction sites removed
- Cloning method Restriction Enzyme
- 5′ cloning site BpiI (destroyed during cloning)
- 3′ cloning site BpiI (destroyed during cloning)
- 5′ sequencing primer CTTTGAGTGAGCTGATACCG
- 3′ sequencing primer GGGTTCCGCGCACATTTC (Common Sequencing Primers)
Addgene and the depositing lab have detected a mixed population at base 2464, which occurs in the promoter region. However, this construct has been functionally verified and should function as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJOG299 was a gift from Johannes Stuttmann (Addgene plasmid # 105360 ; http://n2t.net/addgene:105360 ; RRID:Addgene_105360)
For your References section:Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system. Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J. PLoS One. 2018 May 30;13(5):e0197185. doi: 10.1371/journal.pone.0197185. eCollection 2018. 10.1371/journal.pone.0197185 PubMed 29847550