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pNIC28-Bsa4-ICL1
(Plasmid #105822)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 105822 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    icl1
  • Alt name
    isocitrate lyase
  • Species
    Mycobacterium tuberculosis
  • Promoter T7
  • Tag / Fusion Protein
    • His tag with TEV cleavage (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNIC28-Bsa4-ICL1 was a gift from Ivanhoe Leung (Addgene plasmid # 105822 ; http://n2t.net/addgene:105822 ; RRID:Addgene_105822)
  • For your References section:

    Development of NMR and thermal shift assays for the evaluation of Mycobacterium tuberculosis isocitrate lyase inhibitors. Bhusal RP, Patel K, Kwai BXC, Swartjes A, Bashiri G, Reynisson J, Sperry J, Leung IKH. Medchemcomm. 2017 Oct 17;8(11):2155-2163. doi: 10.1039/c7md00456g. eCollection 2017 Nov 1. 10.1039/c7md00456g PubMed 30108733