PurposehROSA26 targeting CRISPR plasmid
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||105927||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namehROSA26 gRNA
SpeciesH. sapiens (human)
- Promoter U6 promoter
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (destroyed during cloning)
- 3′ cloning site BbsI (destroyed during cloning)
- 5′ sequencing primer aggctgttagagagataattgg (Common Sequencing Primers)
Based on pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene 42230).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:hROSA26 CRISPR-pX330 was a gift from Masato Kanemaki (Addgene plasmid # 105927 ; http://n2t.net/addgene:105927 ; RRID:Addgene_105927)
For your References section:Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble. Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T. Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559. 10.7554/eLife.36559 PubMed 29848445