pCas9
(Plasmid #106396)

• Purpose: Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ
• Depositing Lab: Brian Pfleger
• Publication: Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5.

Backbone

• Vector backbone: pSEVA224
• Vector type: Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: cas9
• Species: Streptococcus pyogenes
• Promoter: J23105

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: na

Gene/Insert 2

• Gene/Insert name: Lambda red genes
• Species: Lambda bacteriophage
• Promoter: araBAD

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: na

Resource Information

• Supplemental Documents:
  • pCas9.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that some discrepancies were found between Addgene's quality control and the depositor's genbank sequence. The depositor noted that these discrepancies do NOT affect plasmid function.