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pGEX-6P1-Vinculin tail
(Plasmid #107154)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 107154 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vinculin
  • Alt name
    VCL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    528
  • Entrez Gene
    VCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL)
  • Promoter Ptac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

GST tag in frame with vinculin tail

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-6P1-Vinculin tail was a gift from Tina Izard (Addgene plasmid # 107154 ; http://n2t.net/addgene:107154 ; RRID:Addgene_107154)
  • For your References section:

    Lipid binding promotes oligomerization and focal adhesion activity of vinculin. Chinthalapudi K, Rangarajan ES, Patil DN, George EM, Brown DT, Izard T. J Cell Biol. 2014 Dec 8;207(5):643-56. doi: 10.1083/jcb.201404128. 10.1083/jcb.201404128 PubMed 25488920