pSB3C5-proB-B0032-E0051
(Plasmid
#107243)
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PurposeExpresses lacZa-GFP fusion from constitutive proB promoter
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107243 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSB3C5
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Backbone manufacturerhttp://parts.igem.org/Part:pSB3C5
- Backbone size w/o insert (bp) 2738
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameProB-RBS(B0032)-Gemini (E0051)
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Alt namesee partsregistry.org for more information
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Insert Size (bp)1500
- Promoter proB
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer tgccacctgacgtctaagaa
- 3′ sequencing primer attaccgcctttgagtgagc (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSB3C5-proB-B0032-E0051 was a gift from Joseph Davis & Robert Sauer (Addgene plasmid # 107243 ; http://n2t.net/addgene:107243 ; RRID:Addgene_107243) -
For your References section:
Design, construction and characterization of a set of insulated bacterial promoters. Davis JH, Rubin AJ, Sauer RT. Nucleic Acids Res. 2011 Feb;39(3):1131-41. doi: 10.1093/nar/gkq810. Epub 2010 Sep 15. 10.1093/nar/gkq810 PubMed 20843779
