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mCherry-eDHFR-Cdc42Q61L
(Plasmid #107267)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 107267 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIVT
  • Backbone manufacturer
    Carmen Williams (Addgene plasmid #32374)
  • Total vector size (bp) 4850
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry-eDHFR-Cdc42Q61L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • Entrez Gene
    CDC42 (a.k.a. CDC42Hs, G25K, TKS)
  • Entrez Gene
    dhfr (a.k.a. D616_p72001)
  • Promoter T7
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Guillaume Halet at Institut de Génétique et Développement de Rennes

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-eDHFR-Cdc42Q61L was a gift from Michael Lampson (Addgene plasmid # 107267 ; http://n2t.net/addgene:107267 ; RRID:Addgene_107267)
  • For your References section:

    Spindle asymmetry drives non-Mendelian chromosome segregation. Akera T, Chmatal L, Trimm E, Yang K, Aonbangkhen C, Chenoweth DM, Janke C, Schultz RM, Lampson MA. Science. 2017 Nov 3;358(6363):668-672. doi: 10.1126/science.aan0092. 10.1126/science.aan0092 PubMed 29097549