pLenti CMV Puro DEST (w118-1) MXRA7
(Plasmid
#107500)
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PurposeExpresses MXRA7, puromycin resistant
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107500 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLenti CMV Puro DEST (w118-1)
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMXRA7 (codon optimized)
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SpeciesH. sapiens (human)
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Insert Size (bp)1329
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MutationCodon optimized sequence
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Entrez GeneMXRA7 (a.k.a. PS1TP1, TMAP1)
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer Dest Rev (GGAGCAACATAGTTA) (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti CMV Puro DEST (w118-1) MXRA7 was a gift from Alejandro Gutierrez (Addgene plasmid # 107500 ; http://n2t.net/addgene:107500 ; RRID:Addgene_107500) -
For your References section:
PRC2 loss induces chemoresistance by repressing apoptosis in T cell acute lymphoblastic leukemia. Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB, Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, Gutierrez A. J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570. 10.1084/jem.20180570 PubMed 30404791
