Addgene - 976 pSG5L HA PTEN 360-364A Plasmid Data

Plasmid 10751: 976 pSG5L HA PTEN 360-364A

Gene/insert name:
PTEN
Insert size:
1200
Species:
H. sapiens (human)
Entrez Gene:
PTEN (10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, TEP1)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Mutation:
360-364A (SSSTS to AAAAA)
Vector backbone:
pSG5L-HA
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4100
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
William Sellers
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Phosphorylation of the PTEN tail regulates protein stability and function. Vazquez et al (Mol Cell Biol. 2000 Jul . 20(14):5010-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10751" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

10750: 800 pSG5L HA PTEN wt

11170: 1055 pSG5L HA PTEN 360-364E