PurposeCan be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||107738||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerJonathan Ting
- Backbone size w/o insert (bp) 7022
- Total vector size (bp) 6091
Modifications to backboneA fragment containing codon-optimized Cre Recombinase, the self-cleaving peptide sequence P2A, and the red fluorescent protein dTomato was swapped into replace SSFO-EYFP-P2A-nlsdTomato in the backbone. Total AAV packaging size (including ITRs and DNA in between) is 3495 bp.
Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
Growth Strain(s)NEB Stable
Gene/Insert nameCre, dTomato
Insert Size (bp)1839
- Promoter hSyn
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer AGTCGAGAAACCGGCTAGAG
- 3′ sequencing primer CCAGAGGTTGATTATCGATA (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe sequence for codon-optimized Cre recombinase was originally published by Rolf Sprengel's lab in Shimshek et al, 2002 (PMID: 11835670) . The sequence for dTomato was originally published by Roger Tsien's lab in Shaner et al, 2008 (PMID: 18454154)
Terms and Licenses
This plasmid can be used to express codon-optimized Cre and, separately, the red fluorescent dTomato. It is useful for studies aiming to fluorescently label cells that have undergone Cre-recombination of a Lox-containing DNA sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-hSyn-Cre-P2A-dTomato was a gift from Rylan Larsen (Addgene plasmid # 107738 ; http://n2t.net/addgene:107738 ; RRID:Addgene_107738)