Addgene - FLAG.PKCepsilon.K/W Plasmid Data

Plasmid 10796: FLAG.PKCepsilon.K/W

Gene/insert name:
PKC epsilon
Insert size:
2100
Species:
H. sapiens (human)
Entrez Gene:
PRKCE (PKCE, nPKC-epsilon)
Fusion protein or tag:
FLAG
Terminal:
N terminal on insert
Mutation:
Kinase dead mutant: K437W (Lys 437 to Trp)
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5446
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Alex Toker
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Regulation of novel protein kinase C epsilon by phosphorylation. Cenni et al (Biochem J. 2002 May 1. 363(Pt 3):537-45. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10796" in your Materials and Methods section.