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Myr.PKCepsilon.FLAG
(Plasmid #10797)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10797 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV6
  • Backbone size w/o insert (bp) 4650
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKC epsilon
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2300
  • Entrez Gene
    PRKCE (a.k.a. PKCE, nPKC-epsilon)
  • Tags / Fusion Proteins
    • Myr (N terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII or KpnI? (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constitutively active. Myr sequence from p60src.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myr.PKCepsilon.FLAG was a gift from Alex Toker (Addgene plasmid # 10797 ; http://n2t.net/addgene:10797 ; RRID:Addgene_10797)
  • For your References section:

    Regulation of novel protein kinase C epsilon by phosphorylation. Cenni V, Doppler H, Sonnenburg ED, Maraldi N, Newton AC, Toker A. Biochem J. 2002 May 1. 363(Pt 3):537-45. 10.1042/0264-6021:3630537 PubMed 11964154