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(Plasmid #108118)


Item Catalog # Description Quantity Price (USD)
Plasmid 108118 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3981
  • Total vector size (bp) 5388
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    Synthetic; chimeric protein
  • Insert Size (bp)
  • Promoter CMV
  • Tag / Fusion Protein
    • ratiometric YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Upcoming article: . Ermakova Y. G., Pak V. V., Bogdanova Y. A., Kotlobay A. A., Yampolsky I.М., Shokhina A. G., A. S. Panova, R. Marygin, D. B. Staroverov, D. S. Bilan, H. Sies and V. V. Belousov “SypHer3s: genetically encoded fluorescent ratiometric probe with enhanced brightness and improved dynamic range”, Chemical Communications, 2017, XX

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC1-SypHer3s was a gift from Vsevolod Belousov (Addgene plasmid # 108118 ; ; RRID:Addgene_108118)
  • For your References section:

    SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range. Ermakova YG, Pak VV, Bogdanova YA, Kotlobay AA, Yampolsky IV, Shokhina AG, Panova AS, Marygin RA, Staroverov DB, Bilan DS, Sies H, Belousov VV. Chem Commun (Camb). 2018 Mar 2. doi: 10.1039/c7cc08740c. 10.1039/c7cc08740c PubMed 29497715