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pC1-SypHer3s-IMS
(Plasmid #108120)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108120 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pC1
  • Backbone manufacturer
    Evrogen
  • Backbone size w/o insert (bp) 3937
  • Total vector size (bp) 5578
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SypHer3s-IMS
  • Species
    Synthetic
  • Insert Size (bp)
    1641
  • Promoter CMV
  • Tag / Fusion Protein
    • ratiometricYFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ermakova Y. G., Pak V. V., Bogdanova Y. A., Kotlobay A. A., Yampolsky I.М., Shokhina A. G., A. S. Panova, R. Marygin, D. B. Staroverov, D. S. Bilan, H. Sies and V. V. Belousov “SypHer3s: genetically encoded fluorescent ratiometric probe with enhanced brightness and improved dynamic range”, Chemical Communications, 2017, upcoming publication

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC1-SypHer3s-IMS was a gift from Vsevolod Belousov (Addgene plasmid # 108120 ; http://n2t.net/addgene:108120 ; RRID:Addgene_108120)
  • For your References section:

    SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range. Ermakova YG, Pak VV, Bogdanova YA, Kotlobay AA, Yampolsky IV, Shokhina AG, Panova AS, Marygin RA, Staroverov DB, Bilan DS, Sies H, Belousov VV. Chem Commun (Camb). 2018 Mar 2. doi: 10.1039/c7cc08740c. 10.1039/c7cc08740c PubMed 29497715