Addgene - pSUPER.PKD3.RNAi Plasmid Data

Plasmid 10818: pSUPER.PKD3.RNAi

Gene/insert name:
PKD3 RNAi
Alt name:
PKD3
Insert size:
64
Species:
H. sapiens (human)
Entrez Gene:
PRKD3 (EPK2, PKC-NU, PKD3, PRKCN, nPKC-NU)
Vector backbone:
pSUPER
(Search Vector Database)
Vector type:
Mammalian Expression, RNAi
Backbone size w/o insert (bp):
3176
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
M13R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Alex Toker
Terms and Licenses:
MTA

Comments:

Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCGTC CTAAGACGGG ACTCTCTTCA AGAGAGAGAG TCCCGTCTTA GGACTTTTTG GAAA-3' and 5'-AGCTTTTCCA AAAAGTCCTA AGACGGGACT CTCTCTCTTG AAGAGAGTCC CGTCTTAGGA CGGG-3'.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Protein kinase D regulates vesicular transport by phosphorylating and activating phosphatidylinositol-4 kinase IIIbeta at the Golgi complex. Hausser et al (Nat Cell Biol. 2005 Sep . 7(9):880-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10818" in your Materials and Methods section.