Addgene - pSUPER.PKCdelta.RNAi Plasmid Data

Plasmid 10819: pSUPER.PKCdelta.RNAi

Gene/insert name:
PKC delta RNAi
Alt name:
PKC delta
Insert size:
64
Species:
H. sapiens (human)
Entrez Gene:
PRKCD (MAY1, PKCD, nPKC-delta)
Vector backbone:
pSUPER
(Search Vector Database)
Vector type:
Mammalian Expression, RNAi
Backbone size w/o insert (bp):
3176
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
M13R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Alex Toker
Terms and Licenses:
MTA

Comments:

Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCGAC AAGATCATCG GCAGATTTCA AGAGAATCTG CCGATGATCT TGTCTTTTTG GAAA-3' and 5'-AGCTTTTCCA AAAAGACAAG ATCATCGGCA GATTCTCTTG AAATCTGCCG ATGATCTTGT CGGG-3'.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Protein kinase Cdelta selectively regulates protein kinase D-dependent activation of NF-kappaB in oxidative stress signaling. Storz et al (Mol Cell Biol. 2004 Apr . 24(7):2614-26. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10819" in your Materials and Methods section.