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pcDNA4/TO/cmycDrosha
(Plasmid #10828)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10828 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA4/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5017
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin (50 ug/ml)
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Drosha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4047
  • GenBank ID
    NM_001100412.1
  • Entrez Gene
    DROSHA (a.k.a. ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4/TO/cmycDrosha was a gift from Thomas Tuschl (Addgene plasmid # 10828 ; http://n2t.net/addgene:10828 ; RRID:Addgene_10828)
  • For your References section:

    The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis. Landthaler M, Yalcin A, Tuschl T. Curr Biol. 2004 Dec 14. 14(23):2162-7. 10.1016/j.cub.2004.11.001 PubMed 15589161