Addgene - pcDNA4/TO/cmycDrosha Plasmid Data

Plasmid 10828: pcDNA4/TO/cmycDrosha

Gene/insert name:
Drosha
Insert size:
4158
Species:
H. sapiens (human)
Entrez Gene:
DROSHA (ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN)
Fusion protein or tag:
myc
Terminal:
N terminal on insert
Mutation:
amino acid changes P1020R and T1230I
Vector backbone:
pcDNA4/TO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5017
Promoter:
CMV
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
CMV Forward List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin (50 ug/ml)
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Zeocin
Sequence: View sequences (5)
Principal Investigator:
Thomas Tuschl
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis. Landthaler et al (Curr Biol. 2004 Dec 14. 14(23):2162-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10828" in your Materials and Methods section.