pCiGSΔΨ
(Plasmid #108312)

• Purpose: Foamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.
• Depositing Lab: David Russell
• Publication: Josephson et al Hum Gene Ther. 2004 Jan;15(1):87-92.

Backbone

• Vector backbone: pCMV beta
• Backbone manufacturer: unknown
• Backbone size w/o insert (bp): 7164
• Total vector size (bp): 5954
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha ; DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gag
• Alt name: pCiGSΔΨ is a newer version of the pCiGS Gag expression plasmid with a more complete deletion in the packaging signal
• Species: Human foamy virus proviral DNA, HSRV2 deletion variant
• Insert Size (bp): 2543
• Mutation: pCiGSΔΨ is a newer version of the pCiGS Gag expression plasmid with a more complete deletion in the packaging signal
• GenBank ID: Y07724.1 Y07723.1
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • Protocol for ΔΦ FV Vector Production

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ΔΦ vectors are “”Deleted Foamy” virus vectors that only retain essential cis-acting viral sequences. The 4 plasmids required to make ΔΦ vectors are pCiES, pCiGSΔΨ, pCiPS, and pΔΦ. The first three plasmids are helper constructs expressing Env, Gag, and Pol respectively. pΔΦ is a deleted foamy vector backbone with a polylinker for you to insert a transgene cassette. A description of the plasmids has been published (Trobridge et al., 2002).

For additional information, please see:
http://depts.washington.edu/dwrlab/reagents/foamy-virus-reagents/
http://depts.washington.edu/dwrlab/reagents/aav-serotype-reagents/