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pcDNA3 HA Arf1 DN T31N
(Plasmid #10833)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10833 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Arf1 T31N
  • Alt name
    Arf1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    550
  • Mutation
    T31N. Defective in GTP binding. Dominant negative.
  • Entrez Gene
    ARF1 (a.k.a. PVNH8)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer bGH PA terminator
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Richard Klausner
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 HA Arf1 DN T31N was a gift from Thomas Roberts (Addgene plasmid # 10833 ; http://n2t.net/addgene:10833 ; RRID:Addgene_10833)
  • For your References section:

    DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism. Furman C, Short SM, Subramanian RR, Zetter BR, Roberts TM. J Biol Chem. 2002 Mar 8. 277(10):7962-9. 10.1074/jbc.M109149200 PubMed 11773070