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pML2(+)BF
(Plasmid #108334)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108334 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Modifications to backbone
    Replacement of ampicillin resistance marker with kanamycin resistance gene and f1 ori from pET-28a(+). Introduction of pheS (T251A/A294G) negative selection marker, and lacZ marker for blue-white screening.
  • Vector type
    Synthetic Biology
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pML2(+)BF was a gift from Andrew Muscroft-Taylor (Addgene plasmid # 108334 ; http://n2t.net/addgene:108334 ; RRID:Addgene_108334)
  • For your References section:

    MIDAS: A Modular DNA Assembly System for Synthetic Biology. van Dolleweerd CJ, Kessans SA, Van de Bittner KC, Bustamante LY, Bundela R, Scott B, Nicholson MJ, Parker EJ. ACS Synth Biol. 2018 Apr 20;7(4):1018-1029. doi: 10.1021/acssynbio.7b00363. Epub 2018 Apr 12. 10.1021/acssynbio.7b00363 PubMed 29620866