Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pML2(+)BF
(Plasmid #108334)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108334 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Modifications to backbone
    Replacement of ampicillin resistance marker with kanamycin resistance gene and f1 ori from pET-28a(+). Introduction of pheS (T251A/A294G) negative selection marker, and lacZ marker for blue-white screening.
  • Vector type
    Synthetic Biology
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pML2(+)BF was a gift from Andrew Muscroft-Taylor (Addgene plasmid # 108334 ; http://n2t.net/addgene:108334 ; RRID:Addgene_108334)
  • For your References section:

    MIDAS: A Modular DNA Assembly System for Synthetic Biology. van Dolleweerd CJ, Kessans SA, Van de Bittner KC, Bustamante LY, Bundela R, Scott B, Nicholson MJ, Parker EJ. ACS Synth Biol. 2018 Apr 20;7(4):1018-1029. doi: 10.1021/acssynbio.7b00363. Epub 2018 Apr 12. 10.1021/acssynbio.7b00363 PubMed 29620866