Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

xCas9 3.7 Citations (5)

Plasmid Article: Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.
Hu JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.
PubMed Journal

Articles Citing xCas9 3.7

Articles
Directed evolution of CRISPR-Cas9 to increase its specificity. Lee JK, Jeong E, Lee J, Jung M, Shin E, Kim YH, Lee K, Jung I, Kim D, Kim S, Kim JS. Nat Commun. 2018 Aug 6;9(1):3048. doi: 10.1038/s41467-018-05477-x. PubMed

Associated Plasmids

Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. PubMed

Associated Plasmids

Genome Editing in Mammalian Cell Lines using CRISPR-Cas. Liu KI, Sutrisnoh NB, Wang Y, Tan MH. J Vis Exp. 2019 Apr 11;(146). doi: 10.3791/59086. PubMed
Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity. Aschenbrenner S, Kallenberger SM, Hoffmann MD, Huck A, Eils R, Niopek D. Sci Adv. 2020 Feb 5;6(6):eaay0187. doi: 10.1126/sciadv.aay0187. eCollection 2020 Feb. PubMed
Precise and broad scope genome editing based on high-specificity Cas9 nickases. Wang Q, Liu J, Janssen JM, Le Bouteiller M, Frock RL, Goncalves MAFV. Nucleic Acids Res. 2021 Jan 25;49(2):1173-1198. doi: 10.1093/nar/gkaa1236. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.