AF20-double-tag
(Plasmid #108389)

• Purpose: Express human PC1
• Depositing Lab: Gregory Germino
• Publication: Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1038/s41598-018-20856-6.

Backbone

• Vector backbone: modified pCI
• Backbone manufacturer: Promega
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PKD1
• Alt name: polycystin 1
• Alt name: PC1
• Species: H. sapiens (human)
• Insert Size (bp): 14481
• Entrez Gene: PKD1 (a.k.a. PBP, PC1, Pc-1, TRPP1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mcherry-TEV cleavage-V5 (N terminal on insert)
  • eGFP-TEV cleavage-S15 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • AF20-double-tag.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note, PKD1 contains P1143A mutation compared to the NCBI reference sequence NP_001009944.2, it is unknown if this mutation affects plasmid function.