p3035 GST-HPV-18 E6
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10850||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHPV18 E6
Insert Size (bp)500
Entrez GeneE6 (a.k.a. HpV18gp1)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer pGEX-5' (Common Sequencing Primers)
Terms and Licenses
See author's map. Please note this differs from the pGEX-2T sequence as indicated.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p3035 GST-HPV-18 E6 was a gift from Peter Howley (Addgene plasmid # 10850 ; http://n2t.net/addgene:10850 ; RRID:Addgene_10850)
For your References section:A cellular protein mediates association of p53 with the E6 oncoprotein of human papillomavirus types 16 or 18. Huibregtse JM, Scheffner M, Howley PM. EMBO J. 1991 Dec . 10(13):4129-35. PubMed 1661671
Map uploaded by the depositor.